Publications
Measuring Integrin Force Loading Rates Using a Two-Step DNA Tension Sensor
J. D. Combs,‡ A. K. Foote,‡ H. Ogasawara, A. Velusamy, Sk A. Rashid, J. N. Mancuso, K. Salaita*
J. Am. Chem. Soc. 2024, ASAP
Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors
Y. Hu, J. Rogers, Y. Duan, A. Velusamy, S. Narum, S. A. Abdullatif & K. Salaita*
Nature Nanotechnology (2024) https://doi.org/10.1038/s41565-024-01723-0
Force-Induced Site-Specific Enzymatic Cleavage Probes Reveal That Serial Mechanical Engagement Boosts T Cell Activation
J. Rogers, R. Ma, A. Foote, Y. Hu, K. Salaita*
J. Am. Chem. Soc. 146, 11, 7233–7242, 2024
Molecular Compressive Force Sensor for Mapping Forces at the Cell–Substrate Interface
S. A. Abdullatif, S. Narum, Y. Hu, J. Rogers, R. Fitzgerald, and K. Salaita*
J. Am. Chem. Soc. 146, 6830–6836, 2024
DNA mechanocapsules for programmable piconewton responsive drug delivery
A. Velusamy, R. Sharma, Sk A. Rashid, H. Ogasawara & K. Salaita*
Nature Communications, 15, 704, 2024
Molecular mechanocytometry using tension-activated cell tagging
R. Ma, Sk A. Rashid, A. Velusamy, B. R. Deal, W. Chen, B. Petrich, R. Li, K. Salaita*
Nat. Methods, available online
2023, https://doi.org/10.1038/s41592-023-02030-7
Force Triggered Self-Destructive Hydrogel
Tharindu Rajasooriya, Hiroaki Ogasawara, Yixiao Dong, Joseph Nicholas Mancuso, Khalid Salaita*
Advanced Materials, Accepted Manuscript
Optomechanically Actuated Hydrogel Platform for Cell Stimulation with Spatial and Temporal Resolution
Allison N. Ramey-Ward, Yixiao Dong, Jin Yang, Hiroaki Ogasawara, Elizabeth C. Bremer, Olga Brazhkina, Christian Franck, Michael Davis, and Khalid Salaita*
ACS Biomaterials Science & Engineering, 9, 5361–5375.
2023
All-Covalent Nuclease-Resistant and Hydrogel-Tethered DNA Hairpin Probes Map pN Cell Traction Forces
Sk Aysha Rashid, Yixiao Dong, Hiroaki Ogasawara, Maia Vierengel, Mark Edoho Essien, and Khalid Salaita*
ACS Applied Materials & Interfaces, 15, 33362–33372.
2023
Polarized focal adhesion kinase activity within a focal adhesion during cell migration
Xiaoquan Li, Joseph Dale Combs III, Khalid Salaita, and Xiaokun Shu
Nature Chemical Biology, https://doi.org/10.1038/s41589-023-01353-y
2023
DNA Nanotechnology for Investigating Mechanical Signaling in the Immune System
Yuesong Hu, Yuxin Duan, Khalid Salaita
Angewandte Chemie, International Edition. https://doi.org/10.1002/anie.202302967
2023
Molecular Mechanocytometry Using Tension-activated Cell Tagging (TaCT)
Rong Ma, Arventh Velusamy, Sk Aysha Rashid, Brendan R. Deal, Wenchun Chen, Brian Petrich, Renhao Li, Khalid Salaita
BioRxiv, 2023, https://www.biorxiv.org/content/10.1101/2023.01.10.523449v1
Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand
Yuxin Duan, Fania Szlam, Yuesong Hu, Wenchun Chen, Renhao Li, Yonggang Ke, Roman Sniecinski, and Khalid Salaita
Nature Biomedical Engineering, 2023, DOI: https://doi.org/10.1038/s41551-023-01114-1
Mechanotransduction at the Cell Surface and Methods to Study Receptor Forces
Rong Ma, Brendan R. Deal and Khalid Salaita
Material-based Mechanobiology from Biomaterials Science Series, Royal Society of Chemistry
eISBN: 978-1-83916-185-8
2022
Mechanically active integrins target lytic secretion at the immune synapse to facilitate cellular cytotoxicity
Mitchell S. Wang, Yuesong Hu, Elisa E. Sanchez, Xihe Xie, Nathan H. Roy, Miguel de Jesus, Benjamin Y. Winter, Elizabeth A. Zale, Weiyang Jin, Chirag Sachar, Joanne H. Lee, Yeonsun Hong, Minsoo Kim, Lance C. Kam, Khalid Salaita, Morgan Huse
Nature Communications, 13, Article number: 3222
2022
Unbreakable DNA tension probes show that cell adhesion receptors detect the molecular force-extension curve of their ligands
Rachel L. Bender, Hiroaki Ogasawara, Anna V. Kellner, Arventh Velusamy, and Khalid Salaita
bioRxiv, preprint
2022
ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion
Tejeshwar C. Rao, Reena R. Beggs, Katherine E. Ankenbauer, Jihye Hwang, Victor Pui-Yan Ma, Khalid Salaita, Susan L. Bellis, and Alexa L. Mattheyses
Journal of Biological Chemistry, 298, 4, 101726
2022
DNA Tension Probes Show that Cardiomyocyte Maturation Is Sensitive to the Piconewton Traction Forces Transmitted by Integrins
Sk Aysha Rashid, Aaron T. Blanchard, J. Dale Combs, Natasha Fernandez, Yixiao Dong, Hee Cheol Cho, and Khalid Salaita
ACS Nano, 16, 4, 5335–5348
2022
Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion
Rao TC and Mattheyses AL
Journal of Visual Experiments, 180, e63529
2022
Molecular Force Sensors
Rachel L. Bender & Khalid Salaita
American Chemical Society (ACS In Focus), eISBN: 9780841299177, DOI: 10.1021/acsinfocus.7e4008
2022
The magnitude of LFA-1/ICAM-1 forces fine tune TCR-triggered T cell activation
Victor Pui-Yan Ma, Yuesong Hu, Anna V. Kellner, Joshua M. Brockman, Arventh Velusamy, Aaron T. Blanchard, Brian D. Evavold, Ronen Alon, and Khalid Salaita
Science Advances, 8, eabg4485
2021
Carfilzomib Treatment Causes Molecular and Functional Alterations of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Parvin Forghani, Aysha Rashid, Fangxu Sun, Rui Liu, Dong Li, Megan R. Lee, Hyun Hwang, Joshua T. Maxwell, Anant Mandawat, Ronghu Wu, Khalid Salaita, and Chunhui Xu
Journal of the American Heart Association, 0:e022247
2021
An Outside-In Swtich in Integrin Signaling Caused by Chemical and Mechanical Signals in Reactive Astrocytes
Leonardo A. Pérez, Aysha Rashid, J. Dale Combs, Pascal Schneider, Andrés Rodríguez, Khalid Salaita and Lisette Leyton
Frontiers in Cell and Developmental Biology, volume 9, article 712627
2021
Mechanically-triggered Hybridization Chain Reaction
Yuxin Duan, Roxanne Glazier, Alisina Bazrafshan, Yuesong Hu, Sk Aysha Rashid, Brian Petrich, Yonggang Ke, and Khalid Salaita
Angewandte Chemie Internal Edition, 60, 19974-19981
2021
Turn-key super-resolution mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
Aaron Blanchard, J. Dale Combs, Joshua Brockman, Anna Kellner, Roxanne Glazier, Hanquan Su, Rachel Bender, Alisina Bazrafshan, Wenchun Chen, M. Edward Quach, Renhao Li, Alexa Mattheyses, and Khalid Salaita
Nature Communications, volume 12, article number 4693
2021
DNA-based microparticle tension sensors (µTS) for measuring cell mechanics in non-planar geometries and for high-throughput quantification
Yuesong Hu, Victor Pui-Yan Ma, Rong Ma, Wenchun Chen, Yuxin Duan, Roxanne Glazier, Brian G. Petrich, Renhao Li, and Khalid Salaita
Angewandte Chemie International Edition, 60, 2-9
2021
DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
Rong Ma, Anna V. Kellner, Yuesong Hu, Brendan R. Deal, Aaron T. Blanchard, Khalid Salaita
Journal of Visualized Experiments, 169, e62348
2021
Multivalent molecular tension probes as anisotropic mechanosensors: concept and simulation
Aaron T Blanchard and Khalid Salaita
Physical Biology, vol. 18 (3), 034001
2021
Spectroscopic Analysis of a Library of DNA Tension Probes for Mapping Cellular Forces at Fluid Interfaces
Roxanne Glazier, Pushkar Shinde, Hiroaki Ogasawara, Khalid Salaita
ACS Applied Materials & Interfaces, vol. 13 (2), pg. 2145–2164
2021
Live-cell super-resolved PAINT imaging of piconewton cellular traction forces
Joshua M. Brockman, Hanquan Su, Aaron T. Blanchard, Yuxin Duan, Travis Meyer, M. Edward Quach, Roxanne Glazier, Alisina Bazrafshan, Rachel L. Bender, Anna V. Kellner, Hiroaki Ogasawara, Rong Ma, Florian Schueder, Brian G. Petrich, Ralf Jungmann, Renhao Li, Alexa L. Mattheyses, Yonggang Ke & Khalid Salaita
Nature Methods, volume 17, pages 1018–1024
2020
Mechanical stimulation of adhesion receptors using light-responsive nanoparticle actuators enhances myogenesis
Ramey-Ward, Allison; Su, Hanquan; Salaita, Khalid
ACS Applied Materials & Interfaces, vol. 12 (32), pg. 35903–35917
2020
EGFR activation attenuates the mechanical threshold for integrin tension and focal adhesion formation
Tejeshwar C. Rao, Victor Pui-Yan Ma, Aaron Blanchard, Tara M. Urner, Shreya Grandhi, Khalid Salaita and Alexa L. Mattheyses
Journal of Cell Science, 133, jcs238840. doi:10.1242/jcs.238840
2020
Variable Incidence Angle Linear Dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles
Aaron Blanchard, Joshua Brockman, Khalid Salaita, and Alexa Mattheyses
Optics Express, Vol. 28, Issue 7, pp. 10039-10061
2020
Forces during cellular uptake of viruses and nanoparticles at the ventral side
Tina Wiegand, Marta Fratini, Felix Frey, Klaus Yserentant, Yang Liu, Eva Weber, Kornelia Galior, Julia Ohmes, Felix Braun, Dirk-Peter Herten, Steeve Boulant, Ulrich S. Schwarz, Khalid Salaita, E. Ada Cavalcanti-Adam & Joachim P. Spatz
Nature Communications, vol. 11, Article number: 32
2020
